PK-15-CAS9 cell line

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PK-15-CAS9 Order

PK-15-CAS9 cell line
Catalog#
YC-A017-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
PK-15-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
PK-15-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Pig
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the PK-15 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a PK-15-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
Porcine Kidney Cell Line Cas9 21.41878700 4.06616783
Porcine Kidney Cell Line h-actin-B 17.35261917
PK-15 Cas9 29.50979614 11.11094475
PK-15 h-actin-B 18.39885139

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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